| TI: |
Title
Pituitary folliculo-stellate-like cell line produces a cytokine-induced
neutrophil chemoattractant |
| AU: |
Author
Zhang, ZX; Koike, K*; Sakamoto, Y; Jikihara, H; Kanda, Y; Inoue,
K; Hirota, K; Miyake, A |
| AF: |
Author Affiliation
Div. Obstetrics and Gynecology, Osaka Univ. Med. Sch., 2-2 Yamadaoka,
Suita, Osaka 565, Japan |
| SO: |
Source
NEUROPEPTIDES, vol. 31, no. 1, pp. 46-51, 1997 |
| IS: |
ISSN
0143-4179 |
| AB: |
Abstract
In previous studies, we reported that cytokine-induced neutrophil
chemoattractant (CINC) was produced in the pituitary gland. Here
we investigated the possibility of detection of CINC immunoreactivity
in the pituitary folliculo-stellate (FS)-like cell line (TtT/GF).
Intense immunoreactivity was observed by immunocytochemistry in
the cytoplasm and cell processes of TtT/GF cells. CINC immunoreactivity
was detected by an enzyme- linked immunosorbent assay in as little
as 3 h after conditioning the medium with TtT/GF cells, and it
increased significantly in a time-dependent manner during the
first 24 h of the culture. This immunoreactivity could be induced
by lipopolysaccharide and tumor necrosis factor- alpha in a time-
and dose-dependent manner. We also observed the expression of
CINC mRNA in TtT/GF cells and LPS increased CINC mRNA accumulation
in TtT/GF cells. These findings indicate that CINC produced by
FS cells may play a role as a paracrine factor of the anterior
pituitary gland, and TtT/GF will provide a useful model system
for studying the regulation of CINC secretion by FS cells. |
| LA: |
Language
English |
| SL: |
Summary Language
English |
| PY: |
Publication Year
1997 |
| PT: |
Publication Type
Journal Article |
| DE: |
Descriptors
pituitary; cytokine-induced neutrophil chemoattractant; immunogenicity;
cell lines; neuroimmunoendocrine system; chemoreception (internal);
CINC protein |
| ID: |
Identifiers
immunogenicity; cell lines |
| CL: |
Classification
N3 11070 Neurochemistry and cellular biology; R 18042 Others;
F 067855 Neuroimmunology |
| SF: |
Subfile
CSA Neurosciences Abstracts; Chemoreception Abstracts; Immunology
Abstracts |
| AN: |
Accession Number
4037051 |
1.8MB
5.5MB
